Figure 3.

ERdj5 Forms Mixed Disulfide Complexes and Noncovalent Interactions with LDLR

(A) Schematic of the domain organization of the LDLR, with the positions of the class 2 mutants used in this study indicated.

(B) ERdj5 C/A or ERdj5 C/A H63Q were immunoisolated with the V5 antibody from cell lysates, separated under reducing (R) or nonreducing (NR) conditions, and immunoblotted for coisolated endogenous LDLR.

(C) Cell lysate from HT1080 cells transiently transfected with HA-tagged LDLR (HA-LR) and ERdj5 (dj5) was immunoblotted with the V5 antibody prior to (left panel) or following (right panel) immunoisolation with the HA antibody.

(D) Cell lysate from HT1080 cells coexpressing HA-LDLR and ERdj5 was immunoblotted with the HA antibody prior to (left panel) or following (right panel) immunoisolation with the V5 antibody.

(E) Same as in (C), except HT1080 cells were cotransfected with HA-LDLR and ERdj5 H63Q.

(F) Same as in (D), except HT1080 cells were cotransfected with HA-LDLR and ERdj5 H63Q.