Table 1. Sequencing and assembly statistics.
Category | Sphingobium indicum B90A | Metagenome (Illumina data) |
---|---|---|
Sequence data | 450 Mbp (320 bp) | 1.6 Gbp (75 bp) |
Reads after quality filtration | 42 044 290 | 20 111 630 |
No. of contigs | 149 (>500 bp) | 1 216 300 (>200 bp) |
Average contig coveragea | 80 | 3 |
Reads used in assembly | 33 635 432 (80%) | 1 608 930(8%) |
N50b | 95 Kbp | 253 bp |
Max. contig size | ∼253 Kbp | ∼3 Kbp |
GC content | 65 | 61 |
Abbreviation: N50 is the length of the smallest contig in a bin that contains largest contigs representing at least half of the total assembly length.
Calculated by mapping of reads to contigs with the criteria of 95% identity over 90% of the read length.
Based on minimum contig length criteria, that is, genome ⩾500 bp and metagenome ⩾200 bp.