Figure 3.

Single-cell characterization of abundant bacterial groups in the cecum by combining FISH and Raman microspectroscopy. Raman spectra were determined for cells from the Bacteroidales (identified using probe Bac-0303) and Lachnospiraceae (using probe Erec-0482) under high-fat (HF) or carbohydrate (CARB) diet. (a) Analysis with the Random Forests classifier revealed clustering of cell spectra by both group and diet on a non-metric multidimensional scaling ordination plot. (b) Mean spectra for each bacterial population and dietary group show that the major discriminative peak (480–482 cm⁻¹) is indicative for storage of polyglucan compounds in Lachnospiraceae.