Figure 4.

Metaproteome analysis (a) Venn diagram showing the occurrence of the 1760 detected microbial proteins. (b) Heat map of the 342 differently detected proteins revealed homogenous patterns within each of the two dietary groups. The normalized spectral abundance factors data set was imported into the R programming environment for statistical computing. (c) The sequences of dietary group-specific proteins were grouped in COG functional categories by protein BLAST. Categories marked with asterisk were significantly different based on statistical analysis of NSAF proportions (P<0.01; two-tailed homoscedastic t-test). The number of proteins used for NSAF calculation is given in brackets (CARB/HF) below the x axis.