Table 2. Identified features contributing to diet-specific FT-ICR-MS profiles.
| CARB intensity (n) | HF intensity (n) | |
|---|---|---|
| CARB diet-specific metabolites | ||
| Steroid hormone biosynthesis | ||
| Allotetra hydrodeoxycorticosterone | 6,022,197 (3) | 0 (0) |
| 11beta,21-Dihydroxy-5beta-pregnane-3,20-dionea | 12,256,770 (3) | 0 (0) |
| Aldosteroneb | 40,386,531 (3) | 0 (0) |
| Cortolc | 19,629,775 (3) | 0 (0) |
| Testosterone glucuronide | 3,626,084 (3) | 0 (0) |
| Androsterone glucuronided | 16,261,771 (3) | 3,900,967 (2) |
| Microbial metabolism in diverse environments | ||
| cis-2-Carboxycyclohexyl-acetic acid | 4,116,462 (3) | 0 (0) |
| cis-2,3-Dihydroxy-2,3-dihydro-p-cumate | 2,408,782 (3) | 0 (0) |
| Biosynthesis of 12-, 14-and 16-membered macrolides | ||
| 10-Deoxymethynolide | 27,444,569 (3) | 3,494,241 (2) |
| 8,8a-Deoxyoleandolide | 59,777,639 (3) | 12,161,516 (3) |
| Oleandolide | 93,629,776 (3) | 18,567,187 (3) |
| 6-Deoxyerythronolide B | 32,516,089 (3) | 3,925,532 (2) |
| Erythronolide B | 63,863,133 (3) | 3,720,904 (2) |
| 3-O-alpha-Mycarosylerythronolide B | 25,751,484 (3) | 0 (0) |
| Bile acid biosynthesis and bile secretion | ||
| Pravastatin | 40,424,347 (3) | 9,377,244 (3) |
| 3alpha,7alpha,12alpha,26-Tetra hydroxy-5beta-cholestane | 4,727,224 (3) | 0 (0) |
| HF diet-specific metabolites | ||
| Fatty acid biosynthesis | ||
| Decanoic acid | 0 (0) | 2,509,582 (3) |
| (6Z,9Z,12Z)-Octadecatrienoic acide | 3,648,269 (2) | 21,133,473 (3) |
| (9Z)-Hexadecenoic acid (=palmitoleic acid) | 5,110,652 (3) | 34,486,283 (3) |
| Linoleatef | 4,116,114 (2) | 49,977,956 (3) |
| Tetradeca noic acid (=myristic acid) | 6,560,960 (3) | 35,976,376 (3) |
| (8Z,11Z,14Z)-Icosatrienoic acid | 7,386,592 (2) | 72,601,368 (3) |
| (9Z)-Octadecenoic acid (=oleic acid) | 42,947,039 (3) | 1,164,910,976 (3) |
| Hexadecanoic acid (=palmitic acid) | 53,440,799 (3) | 856,402,496 (3) |
| Octadecanoic acid (=stearic acid) | 155,914,441 (3) | 1,067,133,440 (3) |
| Porphyrin and chlorophyll metabolism | ||
| D-Urobilinogeng | 0 (0) | 4,375,615 (3) |
| L-Urobilinogen | 1,633,985 (1) | 88,861,815 (3) |
| I-Urobilinogen | 31,249,205 (3) | 592,250,379 (3) |
| L-Urobilin | 26,692,537 (2) | 422,294,496 (3) |
FT-ICR-MS, Fourier-transform ion cyclotron resonance mass spectrometry;
Significant masses were annotated using MassTRIX searching against the KEGG, HMDB and Lipid Map databases. Values are mean peak intensities in cecal samples from mice fed the carbohydrate (CARB) or high-fat (HF) diet. Numbers of positive mice are given in brackets. Superscript letters refer to masses with several possible annotations:
a
17alpha,21-Dihydroxypregnenolone, 3alpha,21-Dihydroxy-5beta-pregnane-11,20-dione.
b
Cortisone.
c
Cortolone.
d
Etiocholan-3alpha-ol-17-one 3-glucuronide.
e
(9Z,12Z,15Z)-Octadecatrienoic acid, Crepenynate.
f
9-cis,11-trans-Octadecadienoate.
g
I-Urobilin.