Frequency of inhibitory interactions among sympatric isolate pairs for each of the seven sampling locations. Differences in the frequency of sympatric inhibitory interactions varied significantly among locations (χ2=28.3; P<0.001). MN1, MN3 and MN5 represent Minnesota 1, 3 and 5, respectively; KS, Konza Prairie; PVB, Panama Volcan Baru; PFS, Panama Fort Sherman and PSC, Panama Santa Clara. There were n=9 to 10 isolates from each location, resulting in a total of n=72–90 sympatric interactions per location (n=612 sympatric interactions total).