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. 2013 Oct 21;16(2):269–279. doi: 10.1111/cmi.12218

Figure 2.

Figure 2

HIF-1α levels remain high in attenuated macrophages even as adhesion and invasiveness decreases.A. Virulent Holstein-Friesian macrophages (H-V) adhere to fibronectin and binding is significantly reduced in attenuated (H-A) macrophages even though attenuated cell spread more than virulent ones.B. H-V macrophages degrade Matrigel better than H-A macrophages.C. Transcription of hif-1α and hif-1β is unchanged in H-V macrophages compared H-A macrophages. Transcript levels were normalized to hprt1 expression. *Student’s t-test, NS P-value > 0.05.D. HIF-1α protein levels also remain largely unchanged in H-V and H-A macrophages. The level of p85 expression was used as a loading control.