Figure 6.

Loss of HIF-1α provokes a shift to more oxidative phosphorylation and reduced macrophage proliferation.A. 16 h treatment of H-V macrophages with increasing concentrations of PX478 results in reduced levels of HIF-1α, HK2 and PKM2 compared with actin.B. Treatment with PX748 at 100 μM for 16 h reduces expression of hif-1a, hk2, pkm2 and ldha.C. 16 h of PX748 at 100 μM initiates a proliferation arrest that becomes pronounced at 48 h.D. Three independent Oroboros measurements of both H-A and H-V macrophages treated for 16 h with PX748 show O₂ fluxes increase and respiration is more mitochondrial, as upon antimycin (AM) inhibition of complex III O₂ flux is strongly diminished. *Student’s t-test, highest P-value = 0.03395 < 0.05.