Table 3.

Primers used to amplify the 14 gene fragments

Name*	Sequence†	AAT (°C)‡
SA-1	G CAC ATG ACC ATI CAR ACY GC	60
SA-4	CC GGT IGC GCA ICC MAR GTT
GI-1	GAT TTG GGI CCI GTY ATG GT	55
GI-2	TG TTG IAI GTA RGC WGG GAA
PG-1	TC AGI GTY GAC TTC AAC GTC CC	55
PG-2	GAA IGT GAA RGC CAT ACC ACC
DE-1	CAC GAT GTI GGT GCT GGT GG	60
DE-2	GC AAC IGG IAC YTG CCA AGG
ME-1	C GAT ATG GTT CAI TAC TTY GGT GA	50
ME-2	AT GGA AAS IAC ITC RGC ATC
AT-1	GCT ATG GAI GGT ACI GAR GG	55
AT-2	AC GGC AGA KGG AAT ACG ACC
GC-1	ATC GGI CAI GTM GCC CAC GG	–
GC-2	GA ACC ACC IGC AAC ACC WCC
FS-1	CAA GAI CAR GGY ATG GGT ATG GA	50
FS-2	TC ACC IAI AGA RTG ACC AGC
PA-1	GGT AAG GGT GGI TCI ATG CA	–
PA-2	AC AGA CAT IGA ITG RCC ACC
VM-1	AAG GIT GTI CAY GAT GGT CA	50
VM-2	GT CAT ICC ITC ACC RAT RGC
EC-1	GYY GGT GGT GGT ATG GG	–
EC-2	GG RCA ACC AGT CAT ICK CAT
OL-1	GAT GTI GCS TCI ATG TAY CC	50
OL-2	GC CAT TTC IAT SGA RTA CCA
LT-1	ATG GAR CCI TGG GAT GGY CC	55
LT-4	GG IGG ATT IGT SAC YTG AGC
LT-5	GCA CCI TGY GAI GGI GCT TG	50
LT-6	CA ATC ITT ACC IGT RTC ACC

^*Odd numbers indicate forward primers; even numbers indicate reverse.

^†I: inosine; otherwise IUPAC codes: R: A/G; W: A/T; M: C/A; K: T/G; Y: T/C; S: C/G.

^‡AAT: alternative annealing temperature. All primers were tested first at 57 °C, and then, most were tested at a second temperature, indicated here.