Table 2.
Sedimentation equilibrium analytical ultracentrifugation analysis for determination of molecular mass (m) in kDa. Rmsd and reduced χ2 are given
| 15.0 μm | 3.0 μm | 0.6 μm | Global analysis of all concentrations | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | m | rmsd | χ2 | m | rmsd | χ2 | m | rmsd | χ2 | m | rmsd | χ2 |
| WT ORF18 ArdA | 39.4 | 0.010 | 4.34 | 36.2 | 0.014 | 7.35 | 38.3 | 0.014 | 8.19 | 37.4 | 0.010 | 6.36 |
| L127E ArdA | 27.3a | 0.007 | 2.12 | 21.2 | 0.005 | 1.03 | 17.0 | 0.005 | 1.15 | 26.7 | 0.010 | 2.37 |
a
The value given is for analysis assuming a single species. However, at the highest concentration, the data fitted best to two species in solution with molecular masses of 39.1 and 20.3 kDa (rmsd = 0.007, χ2 = 1.95). Global simultaneous analysis of all three concentrations assuming a single species was performed with sedphat.