Table 3.
Estimated molecular mass distributions and f/fo values from sedimentation velocity AUC. Rmsd values of sedimentation coefficient (so20,w) and f/fo are given for the major sedimenting species. The rmsd at a confidence level of 0.683 (one standard deviation) is given for the major sedimenting species
| Sample | Concentration (μm) | Measured sedimentation coefficient (so20,w) (% composition) of 260-nm signal | Total% | rmsd | f/fo | ||
|---|---|---|---|---|---|---|---|
| WT ORF18 ArdA | 33.9 | 2.6 (89.0) | 5.2 (4.3) | 9.0 (3.0) | 96.3 | 0.0076 | 1.50 |
| L127E ArdA | 36.9 | 0–0.25 (6.3) | 0.25–0.75 (2.6) | 2.3 (77.7) | 86.6 | 0.0100 | 1.08 |