Figure 2. Prior challenge of mTOR hypomorphic KI mice does not protect them from challenge with live Streptococcus pneumoniae (Pn14).

mTOR KI mice (n = 9) and wild-type littermates (n = 10) were immunized with 1–5×10⁶ CFU live Pn14 and challenged at d14 with 1×10⁸ CFU live Pn14/mouse. A) At day 14 before high dose challenge, KI mice had lower anti-IgG responses (*p<0.05, **p <0.01) than WT mice, B) 15 hours after high dose challenge, more KI mice were bacteremic than WT mice and C) 14 days after high dose challenge, KI mice had a higher rate of mortality than WT mice (p<0.05). The proportions of Ig sequences from WT and KI mice with defined numbers of mutations D,E) were determined. D) Somatic hypermutation (SHM) frequency in immunoglobulin J_H 4 intron (Ig-J_H4) sequences from WT and KI GC (B220⁺GL7⁺Fas⁺) splenic B cells isolated directly from mice immunized with NP-CGG. E) Mutation frequency in immunoglobulin switch μ (Sμ) region from WT and KI splenic CD43⁻ resting B cells stimulated ex vivo with LPS and IL-4 for 120 hrs (IgG₁ induction). The number highlighted in the center of the chart is the total number of sequences analyzed.