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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: Reproduction. 2010 Sep 28;140(6):921–930. doi: 10.1530/REP-10-0268

Figure 1.

Figure 1

Effect of hyperosmolar stress on ID2 protein is independent of MAPK8/9 in TSC, but dependent on PRKAA1/2 in TSC and E3.5 mouse blastocysts. (A) TSCs were cultured in 0 or 400 mM sorbitol with or without the MAPK8/9 inhibitor DJNKI1 (1 μM) for 20 h. Asterisks indicate that the MAPK8/9 inhibitor LJNKL1 has no significant effect on ID2 levels. (B) TSCs were cultured in 0 or 400 mM sorbitol without or with compound C at 1 μM (inhibitor of PRKAA1/2) or SB203580 at 5 μM (inhibitor of MAPK11/14) for 20 h. (C) Embryos were cultured in 0 or 400 mM sorbitol without or with compound C for 2 h. (D) TSCs were incubated with 2 mM AICAR for 0–2 h or with 400 mM for 2 h. (E) TSCs were incubated for 2 h with or without 200 mM sorbitol, with or without 10 μM of the proteasome inhibitor MG132. After treatments, equal amounts of protein were examined with western blots using ID2 and ACTB antibody or amido black. Histograms show the ratio of ID2/ACTB or ID2/amido black band intensity. Error flags are S.D.s for triplicate experiments for TSC or duplicate experiments for embryos.