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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: Reproduction. 2010 Sep 28;140(6):921–930. doi: 10.1530/REP-10-0268

Figure 2.

Figure 2

Rapid transient activation of phosphorylated PRKAA1/2 Thr172 in TSC, ESC, and embryos occurs simultaneously with rapid loss of ID2 protein in TSCs and embryos. Embryos (A and B), TSCs (C and D), and ESC (E) were cultured under optimal conditions, sorbitol was added for 0–90/120 min, and cells and embryos were lysed, proteins fractionated by SDS–PAGE and immunoblotted for PRKAA1/2 Thr172 or ID2 and reprobed for actin (ACTB) or amido black as a loading control. Histograms below each immunoblot show the PRKAA1/2 Thr172 normalized to the loading control and this ratio is set to 1 for unstressed cells/embryos. Y error bars show S.D. of triplicate experiments in TSC and ESC or duplicate experiments in embryos.