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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: Reproduction. 2010 Sep 28;140(6):921–930. doi: 10.1530/REP-10-0268

Figure 3.

Figure 3

Hyperosmolar stress activates PRKAA1/2 over a wide dose range, but ACACA Ser79 is phosphorylated at low doses and ID2 loss and decrease in TSC accumulation occur at high doses. (A) TSCs were plated and cultured for 24 h in media without sorbitol and with 12.5, 25, 50, and 100 mM sorbitol and then counted. (B) TSCs were cultured with the indicated doses of sorbitol for 2 h, fractionated by SDS–PAGE, and immunoblotted and stained for antigens using polyclonal antibodies. All experiments were repeated three times and histograms show X±S.E.M. Superscript ‘a’ indicates significant changes for 0 mM sorbitol stress for cell counts and proteins, and superscript ‘b’ indicates no significant difference for the same unstressed TSCs. Significance was determined by ANOVA followed by Duncan’s post hoc tests. For proteins, the earliest unstressed time point shown was set to 1 and served as a denominator to normalize the experimental values from stressed TSCs.