Fig. 3. Cir1 in the MATα strain influences mating filament formation. Mixtures of cultures from the following strains were carried out on solid V8 medium, V8 medium containing 100 μM FeCl3 or V8 medium containing 100 μM CuSO4: MATα wild-type and MATa wild-type (α × a), MATα cir1Δ#46 and MATa wild-type (αΔ × a), MATα wild-type and MATa cir1Δ#7 (α × aΔ), MATα cir1Δ#46 and MATa cir1Δ#7 (αΔ × aΔ). (A) Each panel represents the periphery of a mixed colony. (B) Higher magnification (× 400) revealed significantly reduced levels of filamentation from a mixture containing MATα cir1Δ. (C) The mixture containing independently generated MATα cir1 mutants (46 and 57) and MAT a cir1 mutants (37) displayed a lack of filamentation at the colony periphery.
