Fig. 3. Induced expression of rice PBZ1 in Arabidopsis causes cell death. Leaves of transgenic (T2-3 and T2-4) and wild type Arabidopsis were infiltrated with DEX (20 μM) for 72 h; T2 stands for 2nd generation whereas the number 3 and 4 are two independent lines. Treated leaves were used for observing cell death morphology and confirmation of enhanced expression of PBZ1. (A) Cell death symptom on leaves as senescence. (B) Binding of trypan blue with cell death tissue of leaves. Senescence leaves of T2-3 and T2-4 stain strongly with trypan blue compared to wild type (WT). (C) Observation of leaves stained with trypan blue under microscopy. (D) Northern blot analysis of total RNA isolated from leaves. (E) Western blot analysis of total protein isolated from leaves.
