Fig. 4. DNA fragmentation and TUNEL analyses of rice SCCs-treated with recombinant PBZ1 protein. (A) DNA fragmentation assay. Rice SCCs were treated with BSA (control; 100 μg/ml), antibody (100 μl anti- PBZ1; control), PBZ1 + antibody [the PBZ1 protein (10 μM) and anti-PBZ1 (100 μl)] or PBZ1 [the PBZ1 protein (10 μM)]. M stands for DNA marker (1.5 kb DNA ladder). (B) TUNEL assay to determine nuclear DNA fragmentation. Rice cultured cells treated for 24 h were used for TUNEL assay. The fluorescent dye DAPI was used for nuclear staining. Control represents the PBZ1 protein (10 μM) plus PBZ1 antibody (100 μl).
