Fig. 6. RNase activity assay of recombinant PBZ1 protein. Total RNA (5 μg) used for this assay was isolated from rice leaf. (A) Dosage- dependent effect of the PBZ1 protein on RNA degradation. (B) Effect of incubation time on RNA degradation using 2.5 μg PBZ1 protein. R.I (50 units) represents RNase inhibitor. Prepared samples were loaded and separated on 0.8% formaldehyde-denatured agarose gel.
