Table 5.

Primers and parameters used in semi-quantitative RT-PCR experiments to confirm the results of the microarray analysis

Gene (Acc No.)	Primer sequences	Annealing temp. (No. of cycles)	Product size (bp)
Ccnb3	5′-CTACTGAGGAACCATCTGTC-3′	53 (23)	280
(NM_183015)	5′-GTGAGCGTCACCTTCCTAAT-3′
Usp9Y	5′-TGTGAGGAATCCAGAGTTG-3′	56 (27)	283
(NM_148943)	5′-CTCTTCTTCATGCTGTGGTG-3′
Agtpbp1	5′-AACGTCAGGAGAGCGGTGGA-3′	56 (29)	188
(NM_023328)	5′-AGAATTTTCATCCCATTCGC-3′
Rabl4	5′-TCTCCGCTAGTACCTGGTTA-3′	58 (25)	177
(NM_025931)	5′-GAACTGGCACTGTCTTCAC-3′
Cds1	5′-CAAGATGGAAGAACTGGtGG-3′	58 (25)	307
(NM_173370)	5′-TGGTAGCGAATGAGGAACTG-3′
GAPDH	5′-CTCACTCAAGATTGTCAGCA-3′	57 (27)	346
	5′-GTCATCATACTTGGCAGGTT-3′