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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: ACS Chem Biol. 2013 Nov 8;9(1):111–115. doi: 10.1021/cb400755b

Figure 1.

Figure 1

The stability of the B-LID domain is regulated by light. a) Schematic of the B-LID domain genetically fused to a protein of interest. Expression of the protein and exposure to blue light results in degradation of the fusion construct. b) NIH3T3 cells stably expressing the YFP-LOV24 fusion protein were treated with either vehicle or 10 μM MG132 and were kept in the dark or irradiated with blue light for 2 hours and analyzed by flow cytometry and immunoblot using anti-YFP antibody. Tubulin is the loading control. The error bars represent the standard deviation of the mean based on at least two experiments. c) NIH3T3 cells expressing the YFP-LOV24 fusion protein were illuminated with blue light, and degradation of the fusion protein was monitored at various times using flow cytometry and anti-YFP immunoblotting. d) Fluorescence micrograph of NIH3T3 cells stably expressing the YFP-LOV24 fusion protein. Cells were kept in the dark or illuminated with blue light for 2 hours. The scalebar represents 100 μm.