Fig. 5.

Viral control is independent of CD25⁺CD4⁺ T cells. (A) Infectious virus within the CNS of individual anti-CD25 and control mAb treated mice. Data represent 2 combined experiments. Line represents the limit of detection. Statistical differences determined by two tailed unpaired t test. *p=<0.05. Bar graphs depict total CD8⁺ T cells (B) and virus specific CD8⁺ T cells (C) per brain. (D) Frequencies of IL-10⁺tetramer⁺ cells within in CNS CD8⁺ T cells. Data are mean±SD and represent 2–4 combined experiments with 3 mice per time point in each experiment. (E) Virus specific cytolytic activity of CNS derived mononuclear cells from mice with or without anti-CD25 treatment. Data are expressed as percentage of specific lysis (mean±SEM) of S510 peptide coated targets at various effector: target (E:T) ratios, adjusted for the frequency of tetramer⁺CD8⁺ T cells. Data are representative of 2 separate experiments with pooled cells from 6–7 mice per group.