Table 2.
Genes which were >3-fold up regulated in TNFα (± FSH)-treated granulosa cells compared with control (± FSH) cells
| Gene symbol |
Fold change |
Gene symbol |
Fold change |
Gene symbol |
Fold change |
|---|---|---|---|---|---|
| (TNFα vs non TNFα treated) | (TNFα vs non TNFα treated) | (TNFα vs non TNFα treated) | |||
|
Cell death | |||||
|
TGM2 |
31 |
XAF1 |
6.8 |
IER3 |
3.6 |
|
CASP4 |
15.2 |
BCL2A1 |
5.3 |
PYCARD1 |
3.3 |
|
IFI6 |
9.2 |
SAMD9 |
4.9 |
TNFSF13B |
3.2 |
|
BEX2 |
8.6 |
EGLN3 |
4.5 |
RIPK4 |
3.1 |
|
BIRC3 |
8.6 |
TRAF1 |
4.3 |
|
|
|
Cell morphology | |||||
|
KRT8 |
10 |
ERP27 |
3.5 |
NCK2 |
3.1 |
|
STMN2 |
7.7 |
PDPN |
3.5 |
TACC1 |
3.1 |
|
MAP2 |
4.7 |
FMNL3 |
3.4 |
GPHN |
3.0 |
|
DCLK1 |
4.5 |
MSN |
3.4 |
MFAP2 |
3.0 |
|
KRT18 |
4.2 |
EMP3 |
3.3 |
|
|
|
Cytokines, hormones and receptors | |||||
|
GPR77 |
61.5 |
CD83 |
6.3 |
PLAUR |
3.8 |
|
CD44 |
21.5 |
ROBO1 |
6.1 |
HLA-DQA1 |
3.7 |
|
IFI44 |
14.9 |
IFIT2 |
6.0 |
NTRK1 |
3.6 |
|
CD19 |
14.2 |
HLA-A |
5.9 |
IL36A |
3.6 |
|
IFI16 |
13.6 |
HLA-DQA2 |
5.9 |
EMR3 |
3.5 |
|
PTGER4 |
13.2 |
IL1RN |
5.9 |
TCRA |
3.5 |
|
CCL2 |
13.1 |
GPR68 |
5.2 |
TGFB1 |
3.5 |
|
SLCO4A1 |
12.3 |
HLA-DMB |
5.0 |
GKN2 |
3.4 |
|
CD200 |
10.8 |
ICAM1 |
4.7 |
IFITM1 |
3.4 |
|
CXCL10 |
10.8 |
HLA-DRA |
4.6 |
HLA-DRB3 |
3.3 |
|
CD40 |
10.1 |
LYVE1 |
4.6 |
FGF2 |
3.2 |
|
IFI27 |
9.5 |
IL1R1 |
4.5 |
VIPR2 |
3.2 |
|
PLXND1 |
9.4 |
NR4A2 |
4.3 |
GPR56 |
3.1 |
|
IFIH1 |
7.1 |
OLR1 |
4.0 |
CD72 |
3.1 |
|
JAG1 |
6.7 |
NRG1 |
3.9 |
IGF2 |
3.1 |
|
CCL5 |
6.4 |
PDGFB |
3.8 |
|
|
|
Extracellular matrix and synthesis | |||||
|
TNC |
52.3 |
COL15A1 |
9.4 |
FMOD |
4.0 |
|
COL6A1 |
14.5 |
COL16A1 |
5.0 |
AGRN |
3.8 |
|
LOXL4 |
8.8 |
COL5A3 |
4.6 |
FN1 |
3.3 |
|
Intercellular and cell to matrix adhesion | |||||
|
BST2 |
6.4 |
THBS3 |
3.6 |
CEACAM8 |
3.2 |
|
VCAM1 |
5.3 |
SPP1 |
3.6 |
CDH1 |
3.1 |
|
CD82 |
4.8 |
CD9 |
3.6 |
|
|
|
ITGA5 |
4.5 |
NUAK2 |
3.4 |
|
|
|
Ion transport | |||||
|
KCNMA1 |
8.9 |
FXYD3 |
3.9 |
CLCA3P |
3.1 |
|
CACNA1G |
6.9 |
GRIA3 |
3.7 |
SLC25A12 |
3.0 |
|
CNGA3 |
4.3 |
TPCN1 |
3.4 |
|
|
|
Proteolysis and inhibition | |||||
|
SLPI |
58.6 |
MMP2 |
5.0 |
CTSH |
3.5 |
|
UBD |
17.1 |
PSMB10 |
3.9 |
TFPI2 |
3.5 |
|
SERPINB2 |
14.9 |
PSMB8 |
3.8 |
TRIM21 |
3.2 |
|
SERPINA5 |
12.5 |
ADAMTSL4 |
3.8 |
UBA7 |
3.2 |
|
ISG15 |
10.8 |
ERAP2 |
3.8 |
PRSS2 |
3.1 |
|
ADAM23 |
8.3 |
USP18 |
3.7 |
CFD |
3.1 |
|
A2M |
5.6 |
TIMP3 |
3.7 |
CSTB |
3.1 |
|
PSMB9 |
5.1 |
C1S |
3.6 |
|
|
|
Transcriptional regulation | |||||
|
GFI1 |
14.1 |
IRF1 |
4.7 |
NFKB2 |
3.7 |
|
NFKBIA |
9.8 |
HEYL |
4.7 |
NFKBIZ |
3.6 |
|
FOXA3 |
9.0 |
HES4 |
4.6 |
H19 |
3.5 |
|
MYB |
8.4 |
IRF8 |
4.2 |
SNAI1 |
3.4 |
|
MIR147 |
5.3 |
LRRFIP1 |
4.0 |
NOSTRIN |
3.4 |
|
TBX3 |
5.2 |
NUPR1 |
4.0 |
DTX1 |
3.3 |
|
BHLHE40 |
4.8 |
RBPMS |
3.9 |
FOXS1 |
3.3 |
|
TCF7 |
4.8 |
IKZF3 |
3.8 |
STAT5A |
3.1 |
|
Transport | |||||
|
RTP4 |
14.5 |
RAB3IP |
3.8 |
SLC37A1 |
3.5 |
|
ABCC3 |
8.0 |
ANKH |
3.7 |
SLCO2A1 |
3.4 |
|
TF |
4.1 |
TAP1 |
3.5 |
SYNGR1 |
3.1 |
|
Other enzymes | |||||
|
PRKCB |
19.3 |
PNPO |
5.0 |
TPST2 |
3.9 |
|
PTGIS |
12.2 |
PIK3CD |
5.0 |
PRKCQ |
3.9 |
|
ALDH1A3 |
11.7 |
GBP4 |
4.8 |
CA5B |
3.8 |
|
OAS2 |
10.5 |
MIOX |
4.7 |
PARP14 |
3.7 |
|
PON3 |
9.3 |
ADCY2 |
4.7 |
PARP12 |
3.7 |
|
DDC |
8.7 |
ADH6 |
4.5 |
LRAT |
3.5 |
|
IDO1 |
7.7 |
UMPS |
4.4 |
CKB |
3.4 |
|
MX2 |
7.5 |
CA2 |
4.4 |
CAMK1D |
3.3 |
|
HSD11B1 |
7.0 |
RSAD2 |
4.3 |
FBP1 |
3.3 |
|
MX1 |
6.6 |
PPP2R3C |
4.3 |
PARP9 |
3.2 |
|
OAS1 |
5.5 |
BCAT2 |
4.3 |
PNKD |
3.2 |
|
ABHD3 |
5.2 |
SQRDL |
4.1 |
CA8 |
3.1 |
|
GLDC |
5.2 |
ISG20 |
4.0 |
|
|
|
XDH |
5.2 |
APOBEC3B |
3.9 |
|
|
|
Other signalling | |||||
|
ENTPD3 |
17.7 |
DNER |
5.8 |
ARHGAP24 |
4.8 |
|
ARHGEF5 |
13.6 |
RASAL1 |
5.2 |
DDX58 |
3.3 |
|
NFKB1A |
9.8 |
TNIP1 |
5.5 |
WNT11 |
3.2 |
|
ARHGAP29 |
8.7 |
ARHGEF11 |
5.1 |
NKG7 |
3.4 |
|
ANXA8L1 |
8.0 |
ARRDC2 |
4.3 |
CDC42EP1 |
3.3 |
|
SLAMF8 |
7.4 |
RGS16 |
4.1 |
PDE4B |
3.5 |
|
TAC3 |
8.7 |
SHISA3 |
4.1 |
IFI30 |
3.2 |
|
GAL |
4.6 |
GBP5 |
4.0 |
RNF128 |
3.0 |
|
DAPP1 |
5.0 |
ARHGDIB |
3.8 |
|
|
|
Other | |||||
|
TREM1 |
11.2 |
TMEM45B |
4.6 |
DPY19L1 |
3.6 |
|
PLAC8 |
7.7 |
MS4A8B |
4.6 |
NELL2 |
3.6 |
|
PARM1 |
6.5 |
SAA3 |
4.6 |
TMEM140 |
3.4 |
|
EPB41L3 |
6.3 |
SELENBP1 |
4.4 |
CCDC85C |
3.4 |
|
FATE1 |
6.2 |
CRYBB1 |
4.4 |
OAF |
3.2 |
|
TRIM47 |
5.4 |
HPCAL1 |
4.2 |
TMED6 |
3.2 |
|
SPARCL1 |
5.4 |
UNC13D |
4.0 |
ABTB2 |
3.2 |
|
NUCB2 |
5.2 |
EPSTI1 |
3.9 |
CRIP2 |
3.2 |
| PDLIM4 | 4.8 | HSPB6 | 3.8 | CSRP2 | 3.1 |
Genes are categorised by function and listed in descending order of fold change in each category. The association of genes with functional terms in this table was determined manually for each gene based on information obtained through the Entrez Gene database of NCBI. The level of fold change and statistical significance ( ≥ 3-fold change, P < 0.05) were determined using the step up Benjamini-Hochberg post-hoc test for multiple corrections following one way ANOVA.