Table 2.
Summary of quantitative neuropathological analysis of microglia
| Brain region |
C57BL/6 J |
PLP-ISEdel |
Two-way ANOVA |
||||
|---|---|---|---|---|---|---|---|
| 2mo | 4mo | 2mo | 4mo | Geno | Age | Inter | |
|
IBA1 IHC | |||||||
| Cerebellum gm |
100 ± 17 |
293 ± 68 |
82 ± 24 |
293 ± 68 |
ns |
** |
ns |
| Cerebellum wm |
100 ± 35 |
424 ± 181 |
235 ± 35 |
1,058 ± 297 |
* |
** |
ns |
| Medulla |
100 ± 21 |
324 ± 127 |
272 ± 41 |
940 ± 216 |
** |
** |
ns |
| Pons |
100 ± 23 |
237 ± 116 |
274 ± 52 |
512 ± 55 |
** |
* |
ns |
| Midbrain |
100 ± 35 |
243 ± 71 |
261 ± 26 |
540 ± 131 |
** |
* |
ns |
| Thalamus |
100 ± 30 |
161 ± 55 |
193 ± 14 |
256 ± 75 |
ns |
ns |
ns |
| Striatum |
100 ± 24 |
323 ± 103 |
87 ± 7 |
335 ± 74 |
ns |
** |
ns |
| Corpus callosum |
100 ± 18 |
195 ± 41 |
152 ± 44 |
294 ± 84 |
ns |
* |
ns |
| Cortex |
100 ± 14 |
143 ± 17 |
81 ± 16 |
136 ± 22 |
ns |
* |
ns |
| Hippocampus |
100 ± 34 |
200 ± 51 |
76 ± 4 |
141 ± 38 |
ns |
* |
ns |
|
CD68 IHC | |||||||
| Cerebellum gm |
100 ± 47 |
500 ± 194 |
375 ± 88 |
1,539 ± 398 |
** |
** |
ns |
| Cerebellum wm |
100 ± 31 |
346 ± 67 |
1,464 ± 127 |
11,607 ± 214 |
**** |
**** |
*** |
| Medulla |
100 ± 11 |
323 ± 35 |
1,571 ± 166 |
7,888 ± 1294 |
**** |
**** |
*** |
| Pons |
100 ± 20 |
433 ± 50 |
1,161 ± 83 |
6,121 ± 1107 |
**** |
**** |
*** |
| Midbrain |
100 ± 21 |
260 ± 26 |
515 ± 76 |
2,960 ± 379 |
**** |
**** |
**** |
| Thalamus |
100 ± 10 |
251 ± 19 |
498 ± 78 |
2,075 ± 257 |
**** |
**** |
**** |
| Striatum |
100 ± 29 |
218 ± 17 |
157 ± 42 |
1,142 ± 213 |
*** |
**** |
** |
| Corpus callosum |
100 ± 15 |
208 ± 22 |
690 ± 96 |
2,715 ± 285 |
**** |
**** |
**** |
| Cortex |
100 ± 25 |
427 ± 115 |
308 ± 62 |
1,301 ± 285 |
*** |
**** |
** |
| Hippocampus |
100 ± 45 |
320 ± 24 |
104 ± 16 |
513 ± 50 |
** |
**** |
** |
|
MHCII IHC | |||||||
| Cerebellum gm |
100 ± 46 |
336 ± 63 |
797 ± 320 |
632 ± 386 |
ns |
ns |
ns |
| Cerebellum wm |
100 ± 75 |
296 ± 156 |
493 ± 275 |
891 ± 223 |
ns |
* |
ns |
| Medulla |
100 ± 40 |
351 ± 57 |
556 ± 281 |
1,551 ± 280 |
* |
* |
ns |
| Pons |
100 ± 53 |
437 ± 100 |
1,587 ± 211 |
963 ± 291 |
*** |
ns |
* |
| Midbrain |
100 ± 33 |
99 ± 22 |
970 ± 250 |
1,139 ± 185 |
**** |
ns |
ns |
| Thalamus |
100 ± 36 |
109 ± 25 |
569 ± 200 |
841 ± 251 |
** |
ns |
ns |
| Striatum |
100 ± 25 |
61 ± 14 |
326 ± 150 |
790 ± 318 |
* |
ns |
ns |
| Corpus callosum |
100 ± 36 |
65 ± 19 |
463 ± 98 |
2,505 ± 1148 |
ns |
ns |
ns |
| Cortex |
100 ± 38 |
197 ± 54 |
398 ± 126 |
474 ± 87 |
** |
ns |
ns |
| Hippocampus | 100 ± 47 | 738 ± 232 | 1,482 ± 692 | 1,677 ± 839 | ns | ns | ns |
2-month-old WT mice used as 100%. Mean ± SEM for each brain region are indicated. (n = 5 to 6 per group). Abbreviations: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; geno = genotype; gm = gray matter; inter = interaction of age and genotype; mo = months-old; ns = not significant; wm = white matter.