Fig. 4.

EphrinA5-Fc treatment does not affect emigration of neural crest cells but reduce cell densities. (A, B) E9.5 wild type neural tubes were isolated and plated into cell culture dishes. After the explants were settled for 5 h, the explants were treated with Fc alone or ephrinA5-Fc. Then, the migration index was measured to compare their outgrowth area. Note that the neural tube explants trea-ted with ephrinA5-Fc show lower cell density in its core area (B, third panel). (C) Quantification of in vitro outgrowth from the neural tube did not reveal significant differences in emigration of neural crest cells (NCCs) irrespective of ephrinA5-Fc treatment (p > 0.5).