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. 2013 Nov 13;11:111. doi: 10.1186/1741-7007-11-111

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Both myofibroblasts (SMA positive) and lipofibroblasts (ADRP positive) were marked by the Tbx4 lung enhancer driven Tet-On system. E18.5 lung tissue sections of triple transgenic mice (Tbx4-rtTA/TetO-Cre/mT-mG) with different Dox induction during early or late gestation as indicated were co-stained with mGFP/ADRP (A) or mGFP/SMA (B). Cell nuclei, counter-stained with DAPI (blue), were not included in the merged panels. ADRP, adipophilin; DAPI, 4',6-diamidino-2-phenylindole; Dox, doxycycline; E, embryonic day; SMA, α-smooth muscle actin.