Table 1.
Bisulfite amplicon sequencing (BSAS) accuracy improvement
| Control (% 5-mC) | Rat | Mouse |
|---|---|---|
| 0 |
5.41 |
30.51 |
| 5 |
2.61 |
1.53 |
| 10 |
1.79 |
10.01 |
| 25 |
3.05 |
1.03 |
| 50 |
12.17 |
25.47 |
| 75 |
7.33 |
40.79 |
| 100 |
3.81 |
4.78 |
| Overall | 5.16864 | 16.3067 |
Mean squared error (MSE) improvements of methylation quantitation from BSAS compared to Sanger/epigenetic sequencing methylation analysis (ESME) from each methylation control are presented. BSAS was more accurate at quantifying methylation at every control, and demonstrated an overall 16-fold decrease in error compared to Sanger/ESME across the mouse controls and 5-fold decrease in error across the rat controls.