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. 2013 Nov 9;31(2):341–354. doi: 10.1093/molbev/mst201

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 2. — Hau-piwi1 and Hau-vasa1 are expressed in gonads of the adult. Horizontal cryosections showing midbody segments of young adult Helobdella, stained with toluidine blue (A, B) or processed by ISH for Hau-piwi1 (C, D) or Hau-vasa1 (E, F). Anterior is up in all panels. For panels A, C, and E, the right side of each section is replaced by a line drawing indicating the reproductive (ovary, testisac) tissues. (A) Helobdella has one pair of ovaries, associated with segment M6, and six pairs of testisacs (five of which are visible here), associated with segments M8–M13. (B) Higher magnification views showing the arrangement of cells within a typical ovary and testisac. (C–F) Equivalent views of specimens processed by ISH show that Hau-piwi1 and Hau-vasa1 are expressed in both tissues. Boxes in (C) and (E) indicate areas shown at higher magnification in (D) and (F), respectively. Scale bar, 200 µm in (A), (C), (E); 50 µm in (B), (D), and (F).