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. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Cytokine. 2013 Dec 2;65(2):202–209. doi: 10.1016/j.cyto.2013.11.002

Fig. 1 — A, Surface expressions of CD1a, Langerin, and E-cadherin (solid lines) demonstrate successful conversion of monocytes into LCs. Dotted lines represent staining with isotype controls. B, A representative example of purified CD3⁺CD25⁻T-cell population in Q4 with purity of 95.1%. C, T-cell proliferation measured using alamarBlue. The mean of six replicates from each individual subject is plotted. The bar represents the mean of the group. P values were calculated using paired t-test.

Fig. 1 — A, Surface expressions of CD1a, Langerin, and E-cadherin (solid lines) demonstrate successful conversion of monocytes into LCs. Dotted lines represent staining with isotype controls. B, A representative example of purified CD3⁺CD25⁻T-cell population in Q4 with purity of 95.1%. C, T-cell proliferation measured using alamarBlue. The mean of six replicates from each individual subject is plotted. The bar represents the mean of the group. P values were calculated using paired t-test.