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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: J Cell Biochem. 2010 Dec 1;111(5):1160–1168. doi: 10.1002/jcb.22828

Fig. 1.

Fig. 1

Purified recombinant LOX-PP attenuates the ERK and AKT kinase activities and downstream signaling targets in MIA PaCa-2 pancreatic cells. A: MIA PaCa-2 cells were treated with 2, 5 μg/ml LOX-PP or water control in DMEM–0.5% FBS for 24 h and then stimulated with 10% FBS for 30 min and WCEs subjected to Western blot analysis for phosphorylated ERK (P-ERK) and AKT (P-AKT), or β-actin, which confirmed equal loading. B: MIA PaCa-2 cells were treated with LOX-PP at the indicated concentrations in DMEM–0.5% FBS for 24 h and then stimulated with 10% FBS for 16 h and nuclear extracts (NEs) were analyzed for the expression of p65, RelB, cyclin D1, or Lamin B.