Fig. 4.

Recovery of disaccharides from brittlestar GAGs digested by different CSases. Equal aliquots (500 ng) of mixed-population brittlestar (A) and A. filiformis (B) GAGs were subjected to different enzyme treatments as described in Figure 3 and recoveries of different disaccharides after complete or selective CSase treatments were recorded as indicated. Monosulfated disaccharides are indicated with filled boxes by sulfate group codes (4S: black, 6S: light grey, 2S: white), disulfated disaccharides are striped in shades of the corresponding component sulfates (4S6S: black/light gray; 2S4S: white/black, 2S6S: white/light gray), trisulfated disaccharides (2S4S6S) are marked as filled dark gray boxes. One of three similar experiments is shown for each GAG sample.