Table II.
Calculated pI of selected membrane-associated GT-B GTs
| GTB-GTa | Length (n° residues) | MW (kDa) | Full pI | NTDb pI | CTDc pI |
|---|---|---|---|---|---|
| TagF | 721 | 85.9 | (313–721) 48.8 kDa 8.14 |
(313–513 + 701–721) 26.5 kDa 9.68 |
(514–700) 22.3 kDa 4.50 |
| WaaA | 353 | 40.7 | (1–353) 40.7 kDa 8.99 |
(1–164 + 336–353) 21.4 kDa 9.65 |
(165–335) 19.3 kDa 8.92 |
| WaaC | 326 | 36.2 | (1–326) 36.2 kDa 8.99 |
(1–162) 18.3 kDa 10.0 |
(163–326) 18.0 kDa 5.67 |
| WaaF | 348 | 39.0 | (1–348) 39.0 kDa 7.15 |
(1–160) 18.4 kDa 9.67 |
(161–348) 20.6 kDa 5.62 |
| WaaG | 374 | 42.3 | (1–374) 42.3 kDa 7.22 |
(1–167 + 358–374) 21.5 kDa 8.35 |
(168–357) 21.1 kDa 5.97 |
| MurG | 355 | 37.8 | (1–355) 37.8 kDa 9.74 |
(1–163 + 341–355) 18.9 kDa 10.38 |
(164–340) 18.9 kDa 7.03 |
| GumK | 400 | 44.4 | (1–400) 44.4 kDa 8.27 |
(1–203 + 361–400) 27.4 kDa 9.22 |
(204–360) 17.0 kDa 6.30 |
| PimA | 386 | 41.2 | (1–386) 41.2 kDa 5.40 |
(1–170 + 348–386) 22.2 kDa 7.11 |
(171–347) 18.9 kDa 4.58 |
aTagF from Staphylococcus epidermidis (pdb code, 3L7I; uniprot code, Q5HLM5_STAEQ), WaaA from Aquifex aeolicus (2XCI, KDTA_AQUAE), WaaC from E. coli (2GT1, E2QGX8_ECOLX), WaaF from E. coli (1PSW, N2I599_ECOLX), WaaG from E. coli (2IW1, RFAG_ECOLI), MurG from E. coli (1F0K, MURG_ECOBW), GumK from Xanthomonas campestris (2Q6V, GUMK_XANCP), PimA from M. smegmatis (2GEK, PIMA_MYCS2).
bNTD, N-terminal domain.
cCTD, C-terminal domain.