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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Microbiol. 2013 Mar 14;15(9):1508–1526. doi: 10.1111/cmi.12124

Figure 1. Identification of the Py-ICP gene and alignment of Py-ICP with its orthologs in other Plasmodium species.

Figure 1

(A) The PY03424 gene from PlasmoDB was re-annotated through RT-PCR and sequencing. The currently annotated PY03424 gene comprises two separate genes: PY03424* and Py-ICP. Introns are shown in yellow and exons are in blue (PY03424*) or red (ICP). (B) The sequence of the re-annotated Py-ICP was aligned with its Pf and Pb orthologs using CLUSTALW. (C) Reverse transcriptase (RT) PCR on Py-ICP mRNA using primers specific for the 5′ and 3′ untranslated regions as designated by arrows in (A). A single band is detected when RT is included in the reaction, but is absent when RT is removed (-RT) and in the non-template control (NTC).