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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Genomics. 2013 Jun 15;102(4):363–371. doi: 10.1016/j.ygeno.2013.06.002

Fig. 3.

Fig. 3

Requirement of Sko1 in glycerol production. (A) RT-qPCR analysis of the glycerol metabolic genes GPD2 and RHR2 in the wt reference strain DAY185, hog1Δ/Δ mutant strain (JMR121), hog1Δ/Δ/+ complemented strain (JMR123), sko1Δ/Δ mutant strain (JMR104), and sko1Δ/Δ/+ complemented strain (JMR109) following 1.0 M NaCl treatment. All genes were analyzed in triplicate. Transcript levels were normalized to TDH3, and fold changes between strains were normalized to the untreated wt strain adjusted to a value of 1.0. (B) Measurements of intracellular glycerol levels following growth in 1.0 M NaCl for the wt, sko1Δ/Δ mutant strain, and the sko1Δ/Δ/+ complemented strain. (C) RTq-PCR analysis of the triacylglycerol lipase genes TGL1 and TGL2. An asterisk indicates a P-value < 0.05 compared to the wt strain in salt.