Figure 2.

Diffusion and energy maps in live neurons. (a) Fluorescence images of cultured neurons expressing mRFP-gephyrin and β^WT-TM-pHluorin. Scale bar: 10 μm. (b and c) Diffusion and energy maps. (d–f) Equivalent set of images and maps for β⁻-TM-pHluorin. (g) Distribution of diffusion coefficients for the membrane constructs β^WT-TM (black), β^S403D-TM (blue), and β⁻-TM (red). (Vertical bars on the x axis) Mean values of the respective distributions. (Inset) Distribution in a lin-log scale. (h) Rugosity of the membrane potential as a function of the region radius. To see this figure in color, go online.