Figure 2.

Fluorescence decay profile for Rhodamine B as a function of temperature. The linearity of the fluorescence decay over three orders of magnitude attests to the single-exponential behavior of Rhodamine B. (Inset) Representation of the Eyring analysis of the temperature-dependent nonradiative rate constant for Rhodamine B (see text for details), which is used to determine the temperature rise in the diffraction-limited confocal volume of the sample. To see this figure in color, go online.