Figure 5.

(a) Diagram of the duplex DNA fluorescent construct used for the dissociation experiments. Green and red stars represent the Cy3 and Cy5 FRET pair used in the experiments. The solid cyan line corresponds to the PEG₁₈ internal linker within the construct to easily allow the construct to form the closed conformation (see Experimental Methods for details). (b and c) ALEX surface image (10 μm × 10 μm) before (b) and after (c) exposure to a pulse of IR light. White circles represent the mask used to measure survival probability (see text for details). To see this figure in color, go online.