Physical parameters of epithelization. (A and B) Closure time tc (solid circles) as a function of the initial effective radius, R0, fitted by Eq. 1 (solid curves) with the constraints D,Rmax ≥ 0. Each circle corresponds to one wound. (A) MDCK wounds. Wild-type (D = 353 ± 38 μm2 h−1, Rmax = 117 ± 11 μm, ), Rho− assay (D = 278 ± 40 μm2 h−1, Rmax = 114 ± 9 μm, N = 30), and Rac− assay (σp/ξ = 198 ± 22 μm2 h−1, Rmax = 105 ± 11 μm, N = 34). (B) HEK-HT assay (σp/ξ = 572 ± 57 μm2 h−1,Rmax = 132 ± 12 μm, N = 653) and HEK-RasV12 assay (σp/ξ = 1531 ± 363 μm2 h−1, Rmax = 223 ± 77 μm, N = 65). (C–F) Epithelization coefficient, D, and cutoff radius, Rmax, for MDCK (C and D) and HEK wounds (E and F). Error bars correspond to a 95% confidence level.