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. 2014 Jan 7;106(1):253–262. doi: 10.1016/j.bpj.2013.11.2959

Figure 2.

Figure 2

FRAP measurements on a 12% w/w pH 3.5 BLG gel, depicted in two columns for FD70 and NaFL respectively. All depicted measurements are performed with 100 ppm probe concentration. (Top) Recovery curves; the fits to the recovery curves are gained in the no-binding limit with D = 6.7 μm2/s and R2 = 0.9996; and D = 24.0 μm2/s and R2 = 0.9941, for FD70 and NaFL, respectively. (Bottom) Diffusion coefficients, obtained using the MLH script, as a function of the number of analyzed postbleach images (two frames per second).