Figure 3.

FRAP measurements on a 12% w/w pH 3.5 BLG gel with 100 ppm NaFL. (a) Recovery curve. full line: FRAPb fit: ${\overline{k}}_{on}=0.16s^{-1}$, $k_{off}=4.8s^{-1}$ and D = 31.4 μm²/s with R² = 0.9990; dashed line: no-binding fit for NaFL as in Fig. 2. (b) Diffusion coefficient, obtained using the FRAPb script, as a function of the number of analyzed postbleach images (two frames per second); (c) Radial Intensity profile of the first postbleach image, with an effective radius of $r_e=28\mu m$.