Comparison of the 16S gene of B-F bacterium with those of B-H (left) and B-S (right) bacteria from synthetic unzipping force signals. Differences, , between basepair free energies of the two bacteria after inferences with the SP (upper) and Box (lower) approximations. Sliding averages over (upper), 10 (lower left), and 1 (lower right) of the differences between the Mfold free energies at 150 mM NaCl are shown for comparison (turquoise line). (Middle) Mismatches between B-F and B-H (left) and between B-F and B-S (right) sequences, obtained from direct alignment of the sequences; there are 102 mismatches between B-F and B-H, and 18 between B-F and B-S. To see this figure in color, go online.