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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: J Surg Res. 2009 Mar 4;156(1):50–56. doi: 10.1016/j.jss.2009.02.004

Figure 3. RNAi rescue for overexpressing cells.

Figure 3

Quantitative analysis of EpCAM expression following transduction with dual expression lentiviral vectors for RNA interference and rescue. Flow cytometry analysis of AGS gastric cancer cells following transduction with dual expression lentiviral vectors. In panels A and B EpCAM is targeted in the ORF or in the 3′ UTR and rescued with EpCAM cDNA resistant to the shRNA. (A) EpCAM KD at open reading frame (ORF) and express RNAi resistant EpCAM (B) EpCAM KD at UTR and express wild type in frame EpCAM coding cDNA (C) EpCAM is targeted in the 3′ UTR and rescued with a truncation mutant of 288 amino acids.