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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: J Surg Res. 2009 Mar 4;156(1):50–56. doi: 10.1016/j.jss.2009.02.004

Figure 5. Expression levels of the second “rescue” transgene are dependent on length from promoter (“positional effect”) in the dual expression lentiviral vector.

Figure 5

(A) MDA-MB-231 cells were transduced with the dual expression lentiviral vectors encoding EpCAM and GFP under the CMV promoter (vectors C and D figure 1): SCR, Rescue-I (CMV-EpCAM-GFP) and Rescue-II (CMV-GFP-EpCAM). (B) EpCAM negative cells HEK-293 cells were transduced with the indicated dual expression lentiviral vectors encoding truncated forms of EpCAM with GFP under the CMV promoter: EpCAM-26, EpCAM-265, EpCAM-314 (C) Mouse cell line IMGE5 cells were transduced with the indicated dual expression lentiviral vectors: SCR, HTLVCMV-IL6-GFP, and HTLV-GFP-IL6. Overexpression of IL-6 by traditional rescue decrease proliferation.