. Author manuscript; available in PMC: 2014 Oct 21.

Published in final edited form as: Chem Res Toxicol. 2013 Sep 12;26(10):1486–1497. doi: 10.1021/tx400213m

Table 2.

Effect of smoking on N7-THBG concentration in human leukocyte DNA

Smokers						Non Smokers

Sample	Gender	Age	Amt. of DNA analyzed (μg)^b	Cigarettes per day	Adducts/10⁹ nucleotides	Sample	Gender	Age	Amt. of DNA analyzed (μg)^b	Adducts/ 10⁹ nucleotides
1	M	52	77.3	11	11.23	14	F	27	93.3	0.79^*
2	F	35	77.8	26	< 0.9	15	M	24	87.2	3.01
3	M	57	82.6	7	2.22	16	F	33	92.7	5.92
4	M	48	38.3	9	5.73	17	F	20	103.0	1.17^*
5	F	19	79.6	23	2.71^*	18	F	34	92.9	< 0.9
6	F	32	73.9	13	6.03	19	M	24	108.2	15.73
7	F	61	78.8	24	19.03	20	F	22	154.5	11.54
8	M	23	89.8	19	6.39	21	M	53	122.9	8.45
9	F	51	101.6	12	14.97	22	F	53	111.7	16.05
10	F	54	107.5	26	10.28	23^a	F	44	102.4	6.83
11	M	43	100.7	29	5.36	24^a	M	28	107.9	0.91^*
12^a	F	33	38.8	19	3.97	25^a	M	28	59.7	7.40
13^a	F	44	42.1	15	10.58	26^a	M	35	35.9	7.14
Mean ± SD		42.5 ± 13.1	144.2 ± 20.8	17.9 ± 7.3	8.20 ± 5.12	Mean ± SD		32.7 ± 11.0	97.9 ± 28.5	7.07 ± 5.29

Sample adduct amount less than LOQ but greater than LOD.

Samples analyzed using an N7-THBG internal standard contaminated with 0.72 fmol of N7-THBG analyte which is finally subtracted from the quantified amount

One third of the total DNA amount was injected on column for HPLC-accurate mass MS/MS analysis.