FIGURE 1:

DC-17γ -MGC, like OCs, are able to make podosomes but not functional SZs for resorption. (A) Cells seeded on a resorbable ACC matrix. As expected, DC-OCs form SZs (F-actin is in white in the left and red in the middle) and are able to resorb bone (resorbed area outlined with a white dotted line, right). DC-17γ -MGCs make dysfunctional SZ structures and are unable to resorb the matrix. Yellow line outlines the cell periphery. Scale bar, 20 μm. (B) Quantification of F-actin–containing structures and resorption areas per cell. Left, percentage of fixed DC-OCs exhibiting SZs compared with DC-17γ-MGCs exhibiting dysfunctional SZ structures. Middle, box-and-whiskers plot showing surface area of SZ and dysfunctional SZs. Represented on the plot are minimum and maximum values (whiskers), mean (+), median (middle line in gray box), and 25 and 75% quartiles (limits of gray box). Right, histogram of the resorption index (area of resorbed matrix per cell) of the two cell types. Error bars are SEM.