FIGURE 10:

Inhibition of cofilin phosphorylation at Ser-3 rescues RhoE^gt/gt bone-resorption defect. Day-5 BM-OCs initially seeded on bone-mimicking OAS were treated for further 48 h with either vehicle (dimethyl sulfoxide) or 50 or 100 nM cucurbitacin E. (A) Immunoblotting showing inhibition of Ser-3–cofilin phosphorylation by cucurbitacin E at 50 and 100 nM without affecting total cofilin level. (B) Micrographs and plot showing resorption index of OCs seeded for 7 d on OAS, including treatment with cucurbitacin E for the last 48 h. Unresorbed matrix is stained with silver nitrate (gray). Resorbed surface is not stained (white). Scale bar, 500 μm.