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. 2014 Jan 30;10(1):e1003442. doi: 10.1371/journal.pcbi.1003442

Figure 5. Validation of novel PTBP1-repressed exons by RT-PCR.

Figure 5

A. Candidate PTBP1-repressed exons with probability greater than 0.65 were validated by RT-PCR following Ptbp1 knockdown. Data shown are averages ± standard error of PSI (Percent of Spliced In) from biological triplicates. Statistical analysis was performed using paired one-tailed Student's t-test (p-values<0.01**, <0.05*). B. Exons with low PTBP1 repression probabilities (≤0.2) were also validated by RT-PCR following Ptbp1 knockdown in biological triplicates.