Figure 6. The effect of mutants on APA-induced ERK activation and GluR1 phosphorylation @845.

A) Subnetwork representing the edges used to generate different crosstalking schemes. A sequence of edge numeric identifiers defines a crosstalking scheme. B) Grey-scale legend, binary encoding, edge-encoding and goodness of fit (r²) for the different crosstalking schemes. The resulting levels of active ERK and GluR1@845 at 15′ after psychostimulant injection do depend on the crosstalking scheme. The scheme 010 is the one closest (r² = 0.8, * label) to the experimental values (dashed red line) for D1R (D) and Golf (E) haploinsufficiency and also for DARPP32 knock out (F). The other crosstalking schemes failed in one or more phenotypes as judged by the low r² (B). With the 010 crosstalking scheme, the model closely matches all the phenotypes (C). The colored dots correspond to the mutant's phenotypes, while the empty circles represent the non-APA phenotypes.