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. 2014 Jan 30;10(1):e1004074. doi: 10.1371/journal.pgen.1004074

Figure 6. Ribosome biogenesis defects in fan mutant zebrafish.

Figure 6

(A) Northern blot analysis of rRNA isolated from 30 and 50 hpf wild type (W) and fan mutants (M) using an oligonucleotide probe against the 5′ETS region of zebrafish pre-rRNA. Pre-rRNAs (a) and (b) are indicated. Methylene blue (MB) staining of the mature 28S and 18S rRNAs was carried out as a loading control. Quantitation of the ration of a/b was performed using Image J. (B) Western blot of whole-cell extracts treated with WDR43 or EGFP control siRNA as indicated. (C) qRT-PCR analyses of human WDR43 gene expression level in non-treated (NT), GFP or WDR43 siRNA treated HeLa cells normalized to ß -actin. (D, E) Subcellular localization of mCherry-tagged Utp15 (red) in control GFP (D) and WDR43 (E) siRNA treated HeLa cells counter stained with DAPI (blue).