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. 2014 Jan 30;8(1):e2664. doi: 10.1371/journal.pntd.0002664

Figure 6. iNOS is involved in Leptospira-induced renal fibrosis.

Figure 6

NO2− production in supernatants from bone marrow macrophages derived from C57BL/6J mice, stimulated for 24 h with live (Lepto) or heat-killed (HKLepto) L. interrogans strain Fiocruz at a multiplicity of infection of 1 (1∶1) 10 (10∶1) and 100 (100∶1), as measured by the Griess reaction. Values are means +SD from 3 separate experiments. (B–E) Bacterial loads in urine (B), renal inflammatory scores (C), percentage of Red Sirius staining (D) and mRNA expression of ACTA-2, fibronectin and Mmp2 (E, upper panel) in kidneys from WT and iNOSko mice infected with 2×108 L. interrogans Fiocruz and sacrificed at day-15 p.i.. Values are means ± SD from WT (n = 3) and iNOSko (n = 5) mice. (E, lower panel), comparison of mRNA expression levels of fibrosis markers in kidneys of naïve WT and iNOSko mice, measured as ΔCT compared to HPRT. Values are means ± SD from WT (n = 3) and iNOSko (n = 3) mice. *P<0.05; **P<0.01 between groups.